2020 Yes https://www.sciencedirect.com/science/article/abs/pii/S0048969720354334?via%3Dihub Fu, Xi Asia Exposure Study Students Middle/Jr High (6-8) Dust Bacteria Fungi Vision Headaches Indoor microbiome Sick building syndrome School Malaysia Bacteria Fungi total, 326 bacterial and 255 fungal genera were detected in dust with large compositional variation among classrooms. Also, half of these samples showed low compositional similarity to microbiome data deposited in the public database. The number of observed OTUs in Gammaproteobacteria was positively associated with SBS (p = 0.004). Eight microbial genera were associated with SBS (p < 0.01). Bacterial genera,
Rhodomicrobium, Scytonema and Microcoleus, were protectively (negatively) associated with ocular and throat symptoms and tiredness, and Izhakiella and an unclassified genus from Euzebyaceae were positively associated with the throat and ocular symptoms. Three fungal genera, Polychaeton, Gympopus and an unclassified genusfrom Microbotryaceae, were mainly positively associated with tiredness. The associations differed with ourprevious study in microbial compounds (endotoxin and ergosterol) and SBS in the same population, in which nasal and dermal symptoms were affected. A higher indoor relative humidity and visible dampness or mould in classrooms were associated with a higher concentration of potential risk bacteria and a lower concentration of potential protective bacteria (p < 0.01). This is the first study to characterize the SBS-associated microorganisms
in the indoor environment, revealing complex interactions between microbiome, SBS symptoms and environmental characteristics.